Ku protein. Mcl-1 directly interacted with the dimeric Ku protein co...

In addition, Ku is an essential protein in humans probably through

May 14, 1999 · The Ku protein binds to DNA ends and other types of discontinuity in double-stranded DNA. It is a tightly associated heterodimer of approximately 70 kDa and approximately 80 kDa subunits that together with the approximately 470 kDa catalytic subunit, DNA-PKcs, form the DNA-dependent protein kinase. … The WRN C-terminal KBM and XLF-like motif bind Ku protein complexes cooperatively. (a) HEK293T cells were co-transfected with expression constructs encoding GFP or the indicated GFP-tagged KBMs and GFP-tagged proteins recovered using GFP-TRAP beads. Aliquots of the input and eluate samples were fractionated by SDS-PAGE and immunoblotted for ...Therefore, Ku protein neddylation is associated with Ku protein ubiquitination, which may induce apoptotic cell death. Astaxanthin, an oxygen-containing carotenoid (xanthophyll), is present in marineThe WRN C-terminal KBM and XLF-like motif bind Ku protein complexes cooperatively. (a) HEK293T cells were co-transfected with expression constructs encoding GFP or the indicated GFP-tagged KBMs and GFP-tagged proteins recovered using GFP-TRAP beads. Aliquots of the input and eluate samples were fractionated by SDS-PAGE and immunoblotted for ...Thus, the Ku protein appears to recognize selectively ends of double-stranded DNA molecules. Possible functions of the Ku autoantigen in eukaryotic cells are …PhD Novel barrier layers for packaging materials suitable for protein-rich foods (ref. BAP-2023-206): We offer a PhD position at the research group of Meat Technology and Science of Protein-Rich Foods (MTSP) at the Ku Leuven, Campus Gent.Fig 1 . Expression levels and localization of Ku protein in normal (MCF10A), transformed (MCF10AT) and cancer (MCF10AC1a) breast cell lines. A) Western blot analysis of Ku70 and Ku86 heterodimer in cytoplasmic, nuclear, and chromatin fractions. 5µg of protein extracts from each cell line were immunoblotted for Ku86 and Ku70.The Ku protein consists of two tightly associated homologous subunits, Ku70 and Ku80, and was originally identified as an autoantigen recognized by the sera of patients with an autoimmunity disease. In eukaryotes, the Ku heterodimer contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by ...Earlier, we have reported that 70 kDa subunit of Ku protein heterodimer (Ku70) binds and inhibits Bax activity in the cytosol and that ubiquitin (Ub)-dependent proteolysis of cytosolic Ku70 ...The Ku protein is a heterodimer composed of 70-kDa (Ku70) and 80-kDa (Ku80) subunits and has the capability of binding selectively to specific forms of DNA [25, 26]. In this capacity, it functions as the regulator of DNA-PK that is active in transcription, DNA recombination, and DNA repair [ 27 - 29 ].Taccioli et al. (1994) showed through genetic and biochemical approaches that the XRCC5 is the 80-kilodalton subunit of the Ku protein. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA ...About this page Ku AND Ki AUTOANTIBODIES HAOYANG ZHUANG, ... WESTLEY H. REEVES, in Autoantibodies (Second Edition), 2007 Ku and DNA-PKcs Antigens The Ku antigen plays an essential role in the DNA non-homologous end-joining reaction [ 6] ( Figure 23.1 ). This pathway prevents cell death or mutations due to double-strand DNA breaks.DNA-dependent protein kinase (DNA-PK) is a serine/threonine protein kinase complex composed of a catalytic subunit (DNA-PKcs) and KU70/80 heterodimer bound to DNA. DNA-PK holoenzyme plays a critical role in non-homologous end joining (NHEJ), the major DNA repair pathway. Here, we determined cryo-ele …The Ku (p70/p80) antigen was first identified and characterized as a DNA binding autoantigen recognized by antibodies present in the sera of certain patients with scleroderma-polymyositis overlap syndrome or systemic lupus erythematosus (SLE) (1).It was later shown to be the DNA binding component of a DNA-dependent protein kinase that phosphorylates several chromatin-bound proteins in vitro ...Nomenclature Ku80 has been referred to by several names including: Lupus Ku autoantigen protein p80 ATP-dependent DNA helicase 2 subunit 2 X-ray repair complementing defective repair in Chinese hamster cells 5 X-ray repair cross-complementing 5 (XRCC5) Epigenetic repressionThe high affinity DNA binding factor (HDF) protein of Saccharomyces cerevisiae is composed of two subunits and specifically binds ends of double-stranded DNA. The 70-kDa subunit, HDF1, shows significant homology with the 70-kDa subunit of the human Ku protein. Like the Ku protein, HDF1 has been show …Ku is a dimeric protein complex that binds to DNA doublestrand break ends and is required for the nonhomologous end joining (NHEJ) pathway of DNA repair. Ku is evolutionarily conserved from bacteria to humans. The ancestral bacterial Ku is a homodimer (two copies of the same protein bound to eachUnlike E. coli, Mycobacterium tuberculosis (Mt) expresses a Ku-like protein and an ATP-dependent DNA ligase that can perform non-homologous end-joining (NHEJ). We have expressed the Mt-Ku and Mt-Ligase D in E. coli using an arabinose-inducible promoter and expression vectors that integrate into specific sites in the E. coli chromosome.E. coli strains have been generated that express the Mt-Ku ...Apr 5, 1993 · Ku, also known as nuclear Factor IV, is an abundant nuclear DNA-binding protein which requires free DNA ends for the initial interaction with double-stranded DNA (dsDNA) and can bind at multiple sites along dsDNA in an energy-independent manner. Ku is thought to function as a molecular scaffold to which other proteins involved in NHEJ can bind, orienting the double-strand break for ligation. The Ku70Oct 21, 2021 · Abstract. Human Ku70 is a well-known endogenous nuclear protein involved in the non-homologous end joining pathway to repair double-stranded breaks in DNA. However, Ku70 has been studied in multiple contexts and grown into a multifunctional protein. In addition to the extensive functional study of Ku70 in DNA repair process, many studies have ... The nuclear pore complex is the key regulator of transport between the cytoplasm and nucleus. Emerging evidence suggests it also regulates gene expression by influencing the internal architecture ...protein kinase complex. Objectives To investigate DNA damage/repair balance and DNA-dependent protein kinase complex in COPD lung and in an animal model of smoking-induced lung damage and to evaluate the effects of oxidative stress on Ku expression and function in human bronchial epithelial cells. Methods Protein expression was quantified …The Ku protein is presumed to bind to some or all of the four DNA ends, perhaps thereby displacing the RAG complex from the two signal ends and allowing their ligation (Fig. 4b) [4], [25]. The Artemis:DNA-PKcs complex, probably recruited to the codings ends by Ku, opens the hairpinned V, D or J ends [10].The Ku protein is a complex of two subunits, Ku70 and Ku80, and was originally identified as an autoantigen recognized by the sera of patients with autoimmune diseases. The Ku protein plays a key role in multiple nuclear processes, e.g., DNA repair, chromosome maintenance, transcription regulation, …Ku proteins function as co-sensors of cGAS to regulate cGAS-STING signaling Summary Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that plays a critical role in regulating antiviral signaling. cGAS binds to DNA and catalyzes the synthesis of cyclic GMP-AMP (cGAMP), which is essential for downstream signal transduction.Human Ku heterodimeric protein composed of Ku70 and Ku80 subunits plays an important role in the non-homologous end-joining DNA repair pathway as a sensor of double strand DNA breaks. Ku is also ...ku gjenden proteinat? USHQIMET QË I PËRMBAJNË Historikisht proteinat janë të lidhura me mishin që në fakt është një burim i mirë bashkë me vitaminat (mes të cilave dhe B12, që gjendet në ushqimet bimore) dhe disa minerale; rekomandohet mish i dobët dhe kufizim në racione gjatë javës, duke zvogëluar konsumin e mishit të ...Collectively, our data show that zinc-finger protein ZNF384 is an adapter of Ku to DNA during DSB repair via cNHEJ. Results. ZNF384 is recruited to DNA damage sites and interacts with NHEJ proteins.Antibodies to the Ku protein, for example, which binds DNA and functions in the repair of DNA doublestranded breaks [160], are found with increased frequency among African-American patients with SLE [161] and are anti-idiotypic to anti-DNA antibodies [162].Anti-Ku antibodies are reported in various connective tissue diseases and the Ku complex can be responsible for a very strong autoimmune answer in autoimmune disease. Nowadays, anti-Ku antibodies are detected by ELISA, counterimmunoelectrophoresis (CIE), immunoblot (IB) and new highly performant tec …Mar 27, 2018 · Aravind, L. & Koonin, E. V. Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system.Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by ...Ku, also known as nuclear Factor IV, is an abundant nuclear DNA-binding protein which requires free DNA ends for the initial interaction with double-stranded DNA (dsDNA) and can bind at multiple ...We found that both Ku proteins interact with the DBD and the hinge region of FXR, and these factors suppress the FXR-mediated BSEP promoter activity and gene expression. Furthermore, Ku proteins associated with FXR on the BSEP promoter. Taken together, these findings suggest that Ku proteins function as corepressors for FXR. Materials and methodsThe eukaryotic Ku protein has key roles in DNA repair and in certain transposition events. Here we show that the Gam protein of phage Mu is conserved in bacteria, has sequence homology with both subunits of Ku, and has the potential to adopt a similar architecture to the core DNA-binding region of Ku.Ku-DNA inhibition does not affect the cellular viability, but it does potentiate the cellular effects of bleomycin. (A) Chemical structures of Ku-DBi's.(B) NSCLC H460 cells were plated and treated 20 μM Ku-DBi as single agents or 1% DMSO as vehicle and incubated for 72 h after which cell viability was determined by CCK-8 assay.(C) Clonogenic survival assay for single agent treatment.Subsequent work established that effective DNA-PKcs recruitment and activation by DNA require Ku, a heterodimer comprising two subunits of ∼70 and 80 kDa (Dvir et al., 1992, ... One such protein is the p53 co-factor hnRNP K, which is phosphorylated by ATM in response to DSBs and protected from MDM2-mediated degradation (Moumen et al., ...The Artemis protein and the Xrcc4/DNA ligase IV heterodimer are then recruited, with Artemis involved in the maturation of the DSB ends and the Xrcc4/DNA ligase IV complex catalysing the re-sealing of the ends [12], [13]. The KU heterodimer is composed of 70–86 kDa subunits, respectively called Ku70 and Ku80 (or Ku86) [14].Scale-bar corresponds to 10 μm. (F) Normalized histograms for the number of Ku AF647 protein dimers bound to λ-DNA (4 μM) at a protein concentration of 33 nM (purple) and a 50 bp blunt-ended DNA substrate (10 nM) in the presence of 120 nM Ku AF647 (green). The histograms were constructed from the number of homodimers at different loci on DNA ...The Ku protein is a heterodimer composed of 70 kD (Ku70) and 80 kD (Ku80) subunits. Ku is the regulatory component of the DNA-dependent protein kinase (DNA-PK) that has a catalytic subunit of approximately 460 kD (DNA-PK(cs)). In this study, the two polypeptides (Ku80/Ku70) of the human Ku were expr …An interaction between Ku70 and Hdm2 was shown by means of immunoprecipitation, whereas none could be shown between 80 kDa subunit of Ku protein heterodimer and Hdm2. Vascular endothelial growth factor (VEGF) is known to inhibit endothelial cell (EC) apoptosis through an Akt-mediated survival kinase signal; however, the mechanism underlying ...Ku protein binds to broken DNA ends and recruits DNA-PKcs to form an enzymatically active complex. To characterize the arrangement of proteins in this complex, we developed a set of photocross ...Anderson C (1993) DNA damage and the DNA-activated protein kinase. Trends Biochem Sci 18:433–437. CrossRef PubMed CAS Google Scholar . Beall E, Admon A, Rio D (1994) A Drosophila protein homologous to the human p70 Ku autoimmune antigen interacts with the P transposable element inverted repeats. Proc Natl Acad Sci USA 91:12681–12685The study suggests that lack of Ku protein leads to radiosensitivity in some tumours and that other factors are responsible for radiosensitive tumours with high Ku expression. It is likely that ...The Ku heterodimer is a well conserved protein(s), with homologs known to exist in every species from bacteria to humans . In all organisms examined, mutations in either Ku subunit result in the expected deficits in DNA DSB repair, DNA recombination, and sensitivities to DNA-damaging agents.ETS1 functions are regulated by protein – protein interactions. [16] [17] In particular, ETS1 protein interacts with several DNA repair proteins. ETS1 binds with DNA-dependent protein kinase (DNA-PK) [where the DNA-PK complex is made up of DNA-PKcs and DNA repair Ku (protein) , and where Ku itself is a heterodimer of two polypeptides , Ku70 ...Ku protein is a heterodimer (Ku70 and Ku86) known to play an important role in V (D)J recombination, apoptosis, telomere fusion, and double-strand break repair. …Ku is capable of binding to hairpin structures, single-stranded nicks, and gaps in an apparently sequence-independent manner (44-46, 61). A number of studies have also suggested that the Ku protein is capable of sequence-specific DNA binding (36-40, 60). Therefore, there appear to be two distinct modes of Ku-DNA interaction: a sequence ...DNA-dependent protein kinase (DNA-PK) is a large protein complex central to the nonhomologous end joining (NHEJ) DNA-repair pathway. It comprises the DNA-PK catalytic subunit (DNA-PKcs) and the heterodimer of DNA-binding proteins Ku70 and Ku80. Here, we report the cryo-electron microscopy (cryo-EM) …The Mt-Ku fusion protein was over-expressed in U2OS cells and this increased the sensitivity of the cells to bleomycin sulfate. Hydrogen peroxide and UV radiation do not predominantly produce DSBs and there was little or no change in sensitivity to these agents. Since in vitro studies were unable to detect binding of Mt-Ku to DNA-PKcs or human ...The Ku protein-DNA-dependent protein kinase system is one of the major pathways by which cells of higher eukaryotes respond to double-strand DNA breaks. The components of the system are evolutionarily conserved and homologs are known from a number of organisms. The Ku protein component binds directly to DNA ends and may help align them for ...In most cells, Ku (an heterodimer of two tightly associated sub-units called Ku70 and. Ku80), is an abondant protein located in the nucleus, where it plays a ...Chinese hamster ovary cell lines lacking XRCC7 showed 10-fold higher sensitivity to radiation and later the protein coded by the gene was identified as DNA-PK [16, 23, 24]. The critical finding that Ku protein is the regulatory component of DNA-PKcs unified both areas of research and gave a new dimension to the NHEJ field.The mixture was excited at 435 nm, and emission was recorded between 440 and 650 nm. One microgram of ECFP-Ku70/EYFP-Ku80 (or a Ku mutant protein) was incubated for 60 minutes in 80 µL FRET buffer (25 mM Tris-HCl pH8.0, 120 mM KCl, 10 mM MgCl 2, and 2 mM DTT). DNA oligonucleotide substrates were prepared in FRET buffer containing 2 μg ...The conserved heterodimer formed between Ku70 and Ku80, known as Ku , is a particularly interesting candidate for the study of regulated protein removal from DNA damage. Ku is the central component of the non-homologous end joining (NHEJ) pathway of DNA double strand break (DSB) repair , and its mode of binding to and high affinity for DSBs ...The Ku protein also has a high affinity to DNA due to its form being preset for the helix. As a result of the asymmetric ring, there is a strong preference (Kd value of 1.5 to 4 X 10^-10 M) for the Ku ring to slide onto the ends of DNA . In addition, other asymmetric features, such as a abundance of Asp residues on the N terminus of the Ku Ring ...The observed effects cannot be accounted for by the differential expression levels of Ku protein in control and SC4 aptamer-expressing cells, but are rather due to augmented DNA binding-capacity of Ku protein, as demonstrated in in vitro studies. Thus, RNA aptamers against Ku protein show potential to sensitize MCF-7 breast carcinoma cells to ...Prism (protein interactions by structural matching) is a system that employs a novel prediction algorithm for protein-protein interactions. It adopts a bottom-up approach that combines structure and sequence conservation in protein interfaces. The algorithm seeks possible binary interactions between …. Ku is a dimeric protein complex that binds to DNA double-strandFat: 0 grams. Carbohydrates: 5 grams. Fiber: 3 gr Ku, also known as nuclear Factor IV, is an abundant nuclear DNA-binding protein which requires free DNA ends for the initial interaction with double-stranded DNA (dsDNA) and can bind at multiple sites along dsDNA in an energy-independent manner. Aug 26, 2005 · The key factors of NHEJ are a May 20, 2023 · Ku protein possesses a ds DNA end binding activity, it also has a major role in V(D)J recombination. The importance of DNA helicases is further strengthened b y the dis­ covery that the Bloom's ... Makanan dengan kandungan protein, karbohi...

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